Journal
/
Biologie
/
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

DOI:
10.3791/1726-v

•

10:52 min

•

February 03, 2010

•

, , ,
1Genetics Training Program,University of Wisconsin-Madison, 2Department of Anatomy,University of Wisconsin-Madison, 3Department of Zoology,University of Wisconsin-Madison, 4Cell and Molecular Biology Training Program,University of Wisconsin-Madison

Chapitres

  • 00:00Titre
  • 00:47Introduction
  • 01:18Assembling Injection Slides and Imaging Slides
  • 02:36Injecting DNA Constructs
  • 04:43Mounting Embryos for Imaging
  • 06:524D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
  • 08:49Behavior of Individual Labeled Neurons and Neural Crest Cells
  • 10:23Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.

Tags

Live ImagingCell MotilityActin CytoskeletonIndividual NeuronsNeural Crest CellsZebrafish EmbryosDevelopmentIn VivoOptical ClarityHigh Resolution ImagingCell BehaviorsMigrationAxon OutgrowthGuidanceMechanismsCell LabelingPlasmid DNA InjectionTransient Mosaic Expression PatternProtrusive ActivityMolecular DynamicsTransgenic LinesFine ProtrusionsChanges In Molecular Distribution

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/1726?article_type=v

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
check_url/fr/1726?article_type=v

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
check_url/fr/1726?article_type=v

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
check_url/fr/1726?article_type=v

Two-Photon-Based Photoactivation in Live Zebrafish Embryos
check_url/fr/1726?article_type=v

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
check_url/fr/1726?article_type=v

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
check_url/fr/1726?article_type=v

Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
check_url/fr/1726?article_type=v

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
check_url/fr/1726?article_type=v

4D Imaging of Protein Aggregation in Live Cells
check_url/fr/1726?article_type=v

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Read Article