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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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Journal JoVE Biologie
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

DOI:
10.3791/1731-v

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10:38 min

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March 03, 2010

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, , ,
1Department of Biochemistry and Biophysics,University of Pennsylvania

Chapitres

  • 00:00Titre
  • 00:27Introduction
  • 01:30General Description of the Oxygen Measurement Protocol
  • 03:30Construction of Phosphorescent Probes
  • 05:31Synthesis of Dendrons
  • 07:16Probe Characterization and Calibration
  • 10:06Conclusion

Summary

Traduction automatique

Traduction automatique

We present principles of oxygen measurements by phosphorescence quenching and review design of porphyrin-based dendritic nanosensors for oxygen imaging in biological systems.

Tags

Phosphorescent NanoprobesOxygen ImagingBiological SystemsPhosphorescence QuenchingProbe DeliveryTriplet StatePhosphorescence LifetimeOxygen ConcentrationMolecular DesignDendritic Phosphorescent NanosensorsPt Or Pd Porphyrin-based Polyarylglycine DendrimersPolyethylene Glycol ResiduesTwo-photon ExcitationAntenna ChromophoresIntramolecular FRETPhotophysical Properties

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