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An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
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Biologie
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Journal JoVE Biologie
An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species

An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species

DOI:
10.3791/1751-v

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08:25 min

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February 22, 2010

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, ,
1Warnell School of Forestry and Natural Resources,University of Georgia (UGA)

Chapitres

  • 00:00Titre
  • 00:23Tissue Harvest
  • 00:45Collection of Phloem
  • 01:07Collection of Secondary Xylem
  • 01:26Collection of Reaction Wood: Compression and Opposite Secondary Xylem
  • 02:21Liquid Nitrogen Storage
  • 02:46RNA Isolation: Tissue Preparation
  • 03:20RNA Isolation Day 1
  • 04:41RNA Isolation Day 2
  • 06:35RNA Precipitation
  • 07:16Final Resuspension and Quantification
  • 08:02Credits

Summary

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Traduction automatique

Many plant tissues, including phloem and xylem from loblolly pine (Pinus taeda L.), contain high levels of phenolics and polysaccharides that interfere with RNA purification. This presentation discusses techniques for the harvest of field-grown tissues and isolation of RNA of sufficient quality for microarrays and other genomic analyses.

Tags

RNA IsolationConifer SpeciesLoblolly PinePinaceae FamilyPhenolic CompoundsPolysaccharidesRNA PurificationOrganic Extraction StepsMicroarray AnalysisGenomic AnalysisField-collected SamplesRNA DegradationTotal RNA YieldsOxidizing AgentsContaminants

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