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Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
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Journal JoVE Neurosciences
Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

DOI:
10.3791/1790-v

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08:46 min

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February 15, 2010

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1STED Microscopy of Synaptic Function,European Neuroscience Institute Göttingen

Chapitres

  • 00:00Titre
  • 00:57Introduction
  • 01:24Preparation of Drosophila melanogaster Neuronal Muscular Junction (NMJ)
  • 02:05Stimulation and FM Staining
  • 02:43Fixing Neuromuscular Junction Preparations
  • 03:19Photoconversion of FM1-43
  • 04:49Processing the Sample for Electron Microscopy
  • 07:29Representative Results
  • 08:19Conclusion

Summary

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Traduction automatique

FM dyes have been of invaluable help in the understanding of synaptic dynamics. FMs are normally followed under the fluorescent microscope during different stimulation conditions. However, photoconversion of FM dyes combined with electron microscopy allows the visualization of distinct synaptic vesicle pools, among other ultrastructure components, in synaptic boutons.

Tags

Synaptic Vesicle PoolsPhotoconversionStyryl DyesExocytosisEndocytosisVesicle RecyclingFluorescently Labeled MarkersOptical TechniquesFM DyeVesicle LabelingNerve StimulationDye LoadingExocytosis ProcessFluorescence Monitoring

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