Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:33Introduction
- 01:14Labeling of hMSCs with Endorem
- 02:24Creating Matrix Associated Stem Cell Implants (MASI)
- 03:28Representative Labeling and Implantation Results
- 03:57Conclusion
Goal of the presentation is to demonstrate a highly reproducible method to generate matrix associated stem cell implants in cartilage defects, which can be visualized with MR imaging. Stem cells are labeled with FDA-approved Ferumoxides, mixed with agarose, implanted into cartilage defects and imaged with a 7T MR scanner.
Tags
ImplantationFerumoxides Labeled Human Mesenchymal Stem CellsCartilage DefectsMatrix Associated Stem Cell ImplantsCartilage RegenerationArthritic Joint InjuriesTraumatic Joint InjuriesAdult Mesenchymal Stem CellsChondrogenic LineageCell-based Articular Cartilage Repair TechnologiesAutologous MSCsCell CulturesDifferentiation PotentialChondrogenic DifferentiationScaffoldExtracellular Matrix ExpansionAgarose ScaffoldHyaline CartilageImmune ResponsesLong-term RetentionIron Oxide NanoparticlesMR Imaging Techniques
