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Harvesting Sperm and Artificial Insemination of Mice
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Journal JoVE Biologie
Harvesting Sperm and Artificial Insemination of Mice

Harvesting Sperm and Artificial Insemination of Mice

DOI:
10.3791/184-v

•

10:00 min

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April 28, 2007

•

,
1Dept. of Biological Chemistry,University of California, Irvine (UCI)

Chapitres

  • 00:06Introduction
  • 01:09Tools and Reagents
  • 02:17Dissection procedure
  • 04:43Removal of epididymis
  • 07:58Visualization of sperm
  • 08:20Insemination of females
  • 09:27Conclusion

Summary

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This protocol demonstrates methods for extracting sperm from the testes of males and then inseminating female mice. This procedure is useful when precise time is needed in developmental studies as well as transgenic work.

Tags

Sperm HarvestingArtificial InseminationMicePeromyscus SpeciesRechercheModelProtocolTestes ExtractionSperm IsolationMilk MixtureFemale Reproductive TractFertilizationEmbryo DevelopmentEmbryo TimingRodent SpeciesMating AssessmentTransgenic WorkReduced Animal UsagePeromyscus Sy

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