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Chapitres
- 00:00Titre
- 00:41Introduction
- 01:13Preparing for Cross-linking
- 03:08Cross-linking and Cell Lysis
- 05:59Immunoprecipitation and Elution of the Bound Proteins
- 09:28IP of Cross-linked Complexes Representative Results
- 10:14Conclusion
Hücre geçirgen çapraz bağlayıcı DSP [dithiobis-(succinimidyl propiyonat)] stabilize geçici ve labil etkileşimleri<em> In vivo</em> Sıkı protein kompleksi arıtma teknikleri kullanarak kendi izolasyon sağlar. Burada immunoprecipitation protein kompleksleri izolasyonu, kültürde yetişen crosslinking hücreler için kullanılan bir tekniktir.
Tags
IsolationLabile Multi-protein ComplexesIn VivoControlled Cellular Cross-linkingImmuno-magnetic Affinity ChromatographyDynamic NatureTransient Protein AssociationsWeak Protein AssociationsLow Affinity InteractionsIsolation MethodsIdentification MethodsProtein NetworksChemical CrosslinkersSelective StabilizationBiochemical LimitationsPurification MethodsProtocolCells In CultureHomobifunctional CrosslinkerSpacer ArmDithiobis-(succinimidyl Proprionate)CleavageReductionDisulphide BondCross-linking And Immunoaffinity ChromatographyMagnetic BeadsProtein ComplexesPurification TechniquesWestern Blot TechniquesMass Spectrometry
