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Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
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Journal JoVE Biologie
Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

DOI:
10.3791/186-v

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14:14 min

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April 29, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI)

Chapitres

  • 00:00Estimation of bead concentrations
  • 00:10Introduction
  • 00:46Reagents
  • 01:27Coating beads with HUVEC cells
  • 03:15Washing beads
  • 05:34Estimation of bead concentrations
  • 08:02Forming the fibrin matrix
  • 10:22Layering lung fibroblasts atop the fibrin gel

Summary

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Traduction automatique

This video demonstrates the protocol of an in vitro angiogenesis assay that recapitulates several stages of angiogenesis. Time-lapse images of sprouting, lumen formation, branching and anastomosis - key features of angiogenesis - are shown.

Tags

Optimized Fibrin Gel Bead AssayStudy Of AngiogenesisMulti-step ProcessAngiogenic StimuliNew VesselsExisting VasculatureDegradation Of Basement MembraneProliferation And Migration Of Endothelial Cells (EC)Extracellular MatrixAlignment Of EC Into CordsBranchingLumen FormationAnastomosisFormation Of New Basement MembraneIn Vitro AssaysMimic Certain Stages Of AngiogenesisVessels In VivoHuman Umbilical Vein ECFibroblastsIn Vitro Angiogenesis AssayCytodex MicrocarriersFibrin GelSoluble FactorsEC SproutingPhase-contrast MicroscopyTime-lapse Microscopy

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