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A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium
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Médecine
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Journal JoVE Médecine
A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

DOI:
10.3791/1861-v

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11:00 min

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June 22, 2010

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, , , , , , , ,
1Department of Medicine, Division of Cardiology,Emory University, 2Coulter Department of Biomedical Engineering,Georgia Tech and Emory University, 3Department of Bioinspired Science,Ewha Womans University

Chapitres

  • 00:00Titre
  • 00:42Introduction
  • 01:24Partial Ligation of the Left Carotid Artery
  • 04:21Carotid Ultrasound Examination to Validate Disturbed Carotid Flow
  • 06:28Intimal RNA Isolation From Carotid
  • 09:19Representative Results
  • 10:11Conclusion

Summary

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Traduction automatique

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Tags

Disturbed Flow-induced AtherosclerosisMouse Carotid ArteryPartial LigationRNA IsolationEndotheliumAtherogenesisLow Flow ConditionsOscillatory FlowCommon Carotid ArteryDirect EvidenceRapid Atherosclerosis DevelopmentApolipoprotein E Knockout MouseEndothelial DysfunctionHyperlipidemic Mouse ModelComplex Lesion FormationIntraplaque NeovascularizationMolecular MechanismsFlow-dependent RegulationVascular BiologyTherapeutic Interventions

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