Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:37Introduction
- 01:10Designing a Yeast Expression System for Type III Effectors
- 02:13Yeast Transformation
- 04:21Preparing Yeast Protein Extracts to Verify Effector Experession
- 06:46Spotting Assay to Detect Effector-induced Growth Inhibition Phenotypes
- 08:08Spotting Assay Results
- 09:02Conclusion
Tags
Growth Inhibition PhenotypesBacterial Type III EffectorsGram-negative Pathogenic BacteriaType III Secretion SystemEffector ProteinsHost CellsImmune ResponsesPathogen GrowthEffector RepertoireVirulenceInfection StagesGenetic ManipulationBudding Yeast Saccharomyces CerevisiaeFunctional Characterization Of Effector ProteinsCellular ProcessesEukaryotes
