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1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology,Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics,Harvard University , 5Department of Applied Mathematics,Harvard University , 6Department of Physics,Massachusetts Institute of Technology, 7Department of Systems Biology,Harvard Medical School, 8Department of Biology,Massachusetts Institute of Technology
Chapitres
- 00:00Titre
- 01:03Introduction
- 02:07Crosslinking, Digestion, Marking of DNA Ends, and Blunt-End Ligation
- 09:46Shearing and Size Selection
- 12:03Biotin Pull-Down and Paired End Sequencing
- 16:38Representative Hi-C Results
- 21:32Conclusion
Hi-C metoden tillåter objektiva, genomet hela identifiering av kromatin interaktioner (1). Hi-C par närhet ligation och massivt parallella sekvensering. Den resulterande data kan användas för att studera genomiska arkitektur på flera skalor: första resultaten identifierade funktioner som kromosom territorier, segregering av öppna och slutna kromatin och kromatinstrukturen på megabase skala.
