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Development of automated imaging and analysis for zebrafish chemical screens.
Journal JoVE
Biologie
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Journal JoVE Biologie
Development of automated imaging and analysis for zebrafish chemical screens.

Development of automated imaging and analysis for zebrafish chemical screens.

DOI:
10.3791/1900-v

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10:49 min

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June 24, 2010

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, , , ,
1Pharmacology and Chemical Biology,University of Pittsburgh Drug Discovery Institute, 2Department of Microbiology and Molecular Genetics,University of Pittsburgh, 3Department of Pharmaceutical Sciences,University of Pittsburgh, 4Department of Chemistry,University of Pittsburgh

Chapitres

  • 00:00Titre
  • 00:53Introduction
  • 01:55Set Up Zebrafish Matings and BCl Treatment
  • 04:47Automated Imaging of 96-Well Plates and Analysis
  • 09:06Representative Results
  • 09:46Conclusion

Summary

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Traduction automatique

We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

Tags

Automated ImagingAnalysisZebrafish Chemical ScreensImage-based High-content ScreeningFGF/RAS/MAPK PathwayZebrafish EmbryosIn Vivo High-content Chemical Screens96-well PlatesHigh Throughput ScreeningAutomated Image Acquisition And AnalysisTransgenic Reporter LineGreen Fluorescent Protein (GFP)Whole Organism ScreensAutomated Imaging And Analysis24-48 Hours Post Fertilization (hpf)Multiwell PlatesSmall MoleculeHyperactivates FGF Signaling

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