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Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
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Journal JoVE Biologie
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

DOI:
10.3791/1932-v

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11:00 min

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May 16, 2010

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1Department of Ophthalmology,SUNY Upstate Medical University

Chapitres

  • 00:00Titre
  • 00:59Introduction
  • 01:24Setting Up for the ACT Assay
  • 03:07Preparing for Microinjection
  • 05:18Microinjection
  • 06:30Isolating Animal Caps from Injected Embryos
  • 07:49Transplantation of Caps onto Flank
  • 09:53Animal Cap Transplant Assay Representative Results
  • 10:34Conclusion

Summary

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Traduction automatique

Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.

Tags

Animal Cap Transplant AssayXenopus LaevisProteinsEmbryonic DevelopmentCell Fate DeterminationNoggin OverexpressionEye FormationComplex PhenotypeEye-specific RoleCell Fate Determination AssayGene ProductsEye Field Transcription Factors (EFTFs)Retinal Cell FateGene Product Identification

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