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La zona subventricolare En-face: colorazione Wholemount e flusso ependimale
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1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, 2Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University
Chapitres
- 00:00Titre
- 00:07Introduction
- 01:02Wholemount Dissection
- 04:14Ependymal Flow Analysis Using Fluorescent Microbeads
- 06:41Fixing and Immunostaining Wholemounts
- 10:58Representative Images and Ependymal Flow Data
- 12:20Conclusion
Le pareti del ventricolo laterale contiene la più grande regione germinale nel cervello adulto dei mammiferi. Tradizionalmente, gli studi sulla neurogenesi in questa regione hanno fatto affidamento su tecniche classiche di sezionamento per l'analisi istologica. Qui vi presentiamo un approccio alternativo, la tecnica wholemount, che fornisce un approccio globale, en-faccia vista di questa regione germinale.
