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Direct Tracheal Instillation of Solutes into Mouse Lung
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Journal JoVE Biologie
Direct Tracheal Instillation of Solutes into Mouse Lung

Direct Tracheal Instillation of Solutes into Mouse Lung

DOI:
10.3791/1941-v

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04:50 min

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August 29, 2010

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, , ,
1Department of Physiology,Emory University, 2Center for Respiratory Health,Emory University, 3Department of Medicine,Emory University

Chapitres

  • 00:00Titre
  • 00:37Introduction
  • 01:16Preparing for Surgery
  • 02:08Surgical Procedure for Intratracheal Instillation
  • 03:46Results of Intratracheal Instillations
  • 04:23Conclusion

Summary

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Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice.

Tags

Direct Tracheal InstillationSolutesMouse LungIntratracheal InstillationsDistal RegionsAlveoliSurvival ProtocolTest SolutesLung TherapeuticsSurfactantsVirusesSmall OligonucleotidesTracheal Instillation ProtocolUpper Respiratory TractHazardous SubstancesNon-invasive ProcedurePhysiological MeasurementsOsmotically BalancedInstillate Volume

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