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Electron Cryotomography of Bacterial Cells
Journal JoVE
Biologie
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Journal JoVE Biologie
Electron Cryotomography of Bacterial Cells

Electron Cryotomography of Bacterial Cells

DOI:
10.3791/1943-v

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14:23 min

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May 06, 2010

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, , , , , , , , , , , , ,
1Division of Biology,California Institute of Technology – Caltech, 2Howard Hughes Medical Institute,California Institute of Technology – Caltech

Chapitres

  • 00:00Titre
  • 00:39Introduction
  • 02:11Plunge Freezing Thin Bacterial Cells
  • 03:41High Pressure Freezing and Cryo Sectioning of Thicker Cells
  • 06:43Examination of the Cells Using the Cryo Light Microscope
  • 07:49Collecting Tilt Series Using the Cryo TEM
  • 09:08Tomogram Reconstruction and Database
  • 10:24Tomogram Segmentation
  • 10:51Movies and Animation
  • 11:22Representative Movies
  • 12:59Conclusion

Summary

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We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

Tags

Electron CryotomographyBacterial CellsCell ShapesCell PolarityGenome SegregationCell DivisionImaging TechnologiesFluorescence Light MicroscopyX-ray CrystallographyNMR SpectroscopyUltrastructure Of CellsThree Dimensions (3D)Macromolecular ResolutionCryo Transmission Electron Microscope (cryoTEM)Low Temperature ImagingVitreous StateCryogensEthaneEthane/propane MixturesHigh-pressure FreezingCryo-sectioningTwo-dimensional Projection ImagesTomogram

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