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Eukaryotic Polyribosome Profile Analysis
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Biologie
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Journal JoVE Biologie
Eukaryotic Polyribosome Profile Analysis

Eukaryotic Polyribosome Profile Analysis

DOI:
10.3791/1948-v

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09:16 min

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June 15, 2010

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, , , , , , ,
1Department of Molecular Genetics, Microbiology, and Immunology,University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School

Chapitres

  • 00:45Introduction
  • 01:15Preparation of Sucrose Gradients
  • 02:53Preparation of Extracts from Yeast Cells
  • 05:06Centrifugation of Gradients and Collection of Data and Fractions
  • 08:35Representative Trace of a Ribosome Extract
  • 08:56Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a protocol for the extraction of translating ribosomes from eukaryotic cells. Once extracted, ribosomes are separated into monosomes and polyribosomes by sucrose gradient fractionation to allow different ribosomal populations to be analyzed. As such, this method is the gold standard for examining the regulation of translation.

Tags

EukaryoticPolyribosome Profile AnalysisProtein SynthesisTranslation RegulationMRNA/ribosome ComplexesSucrose Density GradientPolyribosomesMonosomesTranslation InitiationElongation/terminationMRNA CohortExperimental ConditionsRibosome AssemblySmall Ribosomal SubunitLarge Ribosomal Subunit

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