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Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
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Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression

DOI:
10.3791/1952-v

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10:24 min

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June 11, 2010

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, ,
1Department of Cell Biology and Molecular Genetics,University of Maryland College Park

Chapitres

  • 00:00Titre
  • 01:00Introduction
  • 01:35Floral-dip Transformation of Arabidopsis thaliana
  • 05:20Selecting for Transgenic Plants
  • 07:25Examining pTS02::GUS Expression Patterns
  • 09:26Representative Images of GUS Expression in Arabidopsis thaliana Transformants
  • 09:57Conclusion

Summary

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This article illustrates the floral-dip method of Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana. By introducing a cell-cycle regulated promoter-reporter, pTSO2::β-glucuronidase (GUS), into Arabidopsis, we illustrates how one detects GUS reporter expression in transgenic seedlings.

Tags

Floral-dip TransformationArabidopsis ThalianaPTSO2::β-glucuronidase Reporter Gene ExpressionForeign GenesModern BiologyBiotechnologyAgrobacterium TumefaciensPlasmid ConstructsArabidopsis ResearchPlant Gene FunctionReporter Geneβ-glucuronidase (GUS)TSO2 PromoterRibonucleotide Reductase (RNR)DNDP BiosynthesisS-phase Of The Cell CycleTransgenic Arabidopsis SeedlingsEnseignement

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