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In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
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Biologie
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Journal JoVE Biologie
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

DOI:
10.3791/1969-v

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07:42 min

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March 19, 2010

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, , , , ,
1Institute of Biophysics,Johannes Kepler Universitat Linz

Chapitres

  • 00:00Titre
  • 00:07Introduction
  • 02:17μ-contact Printing
  • 04:15Microscopy
  • 05:50Data Analysis
  • 06:27Representative Results
  • 06:56Conclusion

Summary

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This video shows experiments with subsequent analysis of protein-protein interactions by the use of micro-patterned surfaces. The approach offers the possibility to detect protein interactions in living cells and combines high throughput capabilities with the possibility to extract quantitative information.

Tags

In-vivo DetectionProtein-protein InteractionsMicro-patterned SurfacesInteraction NetworkMoleculesCell FunctionMetabolismMethodological OptionsMolecular InteractionsQuantitativeHigh ThroughputHigh-end Quantitative ApproachesProtein-protein Interactions SystemFluorophore-labeled ProteinPrey And Bait InteractionsMicropatterned SurfacesAntibodiesBait Exoplasmic DomainFluorescent Prey RedistributionHigh SensitivityWeak InteractionsScreening Tool

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