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Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
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Journal JoVE Biologie
Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR

Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR

DOI:
10.3791/1974-v

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11:22 min

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May 04, 2010

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, , ,
1Department of Entomology,The Ohio State University

Chapitres

  • 00:00Titre
  • 00:50Introduction
  • 01:56Larval Dissection
  • 04:03RNA Extraction
  • 07:19qRT-PCR
  • 09:24Representative Results for Larval EAB Dissection and qRT-PCR
  • 10:38Conclusion

Summary

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Quantitative real-time PCR (qRT-PCR) is an effective tool to diagnose mRNA levels in different insect tissues and developmental stages. In this report we show the use of qRT-PCR to ascertain mRNA levels in different larval tissues and developmental stages of the invasive insect species, emerald ash borer.

Tags

Gene ExpressionEmerald Ash BorerAgrilus PlanipennisQuantitative Real-time PCREABInvasive PestAsh TreesMolecular KnowledgePhysiological ProcessesMRNA LevelsDevelopmental StagesPeritrophin GeneAP-PERI1Ribosomal Protein AP-RP1Peritrophic Membrane/matrixInsect Gut

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