/
/
Imaging in vivo del transgenico parassiti Leishmania in un host live
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3Department of Internal Medicine,University of Iowa, 4Department of Molecular Microbiology,Washington University School of Medicine, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Chapitres
- 00:04Titre
- 00:54Introduction
- 01:37Infection of Mice with Leishmania
- 03:54Bioluminescent Imaging
- 06:18Data Analysis
- 07:04Representative Results
- 09:17Conclusion
Un<em> In vivo</em> Sistema di imaging viene utilizzato per generare misurazioni quantitative di infezione murino con il protozoo Trypanosomatid<em> Leishmania</em>. Questo è un metodo non invasivo e non letali per il rilevamento di parassiti esprimere luciferasi in molti tessuti nel corso della cronica<em> Leishmania</em> Spp. infezione.
Tags
In Vivo ImagingTransgenic Leishmania ParasitesLive HostLeishmania SpeciesVisceral LeishmaniasisCutaneous LeishmaniasisMouse ModelsParasite BurdensMicroscopyLimiting Dilution AssayQPCR AmplificationIn Vivo Imaging System (IVIS)Bioluminescent SignalLuciferase ExpressionIntradermal InjectionIntravenous InjectionLongitudinal StudiesInter-animal VariationParasite InoculaMultiplication Of ParasitesHigh SensitivityCutaneous Locations
