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Biblioteksoptagelse, biotin-pull-down og PCR-amplifikation ved hjælp af knapt celle-DNA-bibliotek
JoVE Journal
Genetics
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JoVE Journal
Genetics
Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library
Biblioteksoptagelse, biotin-pull-down og PCR-amplifikation ved hjælp af knapt celle-DNA-bibliotek
DOI:
10.3791/200140-v
•
02:37 min
•
April 21, 2023
•
Llorenç Rovirosa*
1
,
Laureano Tomás-Daza*
1,2
,
Blanca Urmeneta
,
Alfonso Valencia
3
,
Biola M. Javierre
4
1
Josep Carreras Leukaemia Research Institute
,
2
Barcelona Supercomputing Center
,
3
Catalan Institution for Research and Advanced Studies (ICREA)
,
4
Institute for Health Science Research Germans Trias i Pujol
Tags
DNA Library
Scarce Cell DNA
Biotin Pull-down
PCR Amplification
Library Capture
Streptavidin Beads
Binding Buffer
Wash Buffer
DNA Purification
Paramagnetic Beads
Automated Electrophoresis
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