Journal
/
Biologie
/
Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

DOI:
10.3791/2002-v

•

13:10 min

•

September 08, 2010

•

, ,
1Department of Biology,University of Rochester

Chapitres

  • 00:00Titre
  • 01:13Introduction
  • 02:22The Reporter Constructs
  • 05:02Integration of the Reporter Constructs and Induction of DSBs
  • 09:18Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
  • 11:34Representative FACS Results of the DSB Repair Assay
  • 12:31Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes GFP-based fluorescence in vivo assays that separately quantify homologous recombination and nonhomologous end joining in mammalian cells.

Tags

DNA Double-strand BreakRepairMammalian CellsNonhomologous End JoiningNHEJHomologous RecombinationHRPerturbationsPremature AgingTumorigenesisQuantitative MeasurementFluorescent Reporter ConstructsGFP GeneI-SceI EndonucleaseInduction Of DSBsGFP Positive CellsFlow Cytometry

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2002?article_type=v

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
check_url/fr/2002?article_type=v

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
check_url/fr/2002?article_type=v

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
check_url/fr/2002?article_type=v

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
check_url/fr/2002?article_type=v

Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
check_url/fr/2002?article_type=v

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
check_url/fr/2002?article_type=v

Analyse av Cell Cycle Posisjon i pattedyrceller
check_url/fr/2002?article_type=v

Real-time Imaging av Enkelt Engineered RNA transkripsjoner i levende celler ved hjelp Proporsjonellekspansjon bimolekylære Beacons
check_url/fr/2002?article_type=v

Visualisering av miniSOG Tagged DNA Repair Proteiner i kombinasjon med Electron Spektroskopiske Imaging (ESI)
check_url/fr/2002?article_type=v

Undersøkelse av Proteiner Bundet til begynnende DNA i pattedyrceller Bruke BrdU-chip-Slot-vestlig Technique

Read Article