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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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Etablering och passage av tunntarmsorganoider

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Establishment and Passaging of Small Intestine Organoids

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Etablering och passage av tunntarmsorganoider

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

DOI:

10.3791/200273-v

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05:32 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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Keywords: Intestinal Organoids Small Intestine Rat Dissection Intestinal Fragment Mesentery PBS EDTA Epithelium Villi Crypts Cell Culture

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