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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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从3D类器官生成大鼠肠道2D单层

Journal JoVE
Biologie

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Journal JoVE Biologie

Generation of Rat Intestinal 2D Monolayers from 3D Organoids

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从3D类器官生成大鼠肠道2D单层

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

DOI:

10.3791/200274-v

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02:47 min

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June 23, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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Keywords: Rat Intestinal 2D Monolayers 3D Organoids EME Coating Collagen Coating Organoid Disruption Trypsin Treatment RIOM2D Y27632 2D Monolayers 3D Structures

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