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Immunologie et infection

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Staining and Imaging to Visualize Neutrophil Extracellular Traps

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组织样本中的自发荧光阻断和抗原修复

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Immunologie et infection

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Journal JoVE Immunologie et infection

Autofluorescence Blocking and Antigen Retrieval in Tissue Samples

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组织样本中的自发荧光阻断和抗原修复

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:

10.3791/200336-v

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01:56 min

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August 18, 2023

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Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

Tags

Autofluorescence Blocking Antigen Retrieval Citrate Buffer Target Retrieval Solution TRS PH Deionized Water Heat Treatment Wet Chamber Autofluorescence-reducing Agent Ethanol Antigen Retrieval TRIS Buffered Saline Tween Hydrophobic Barrier Pen

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