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Bio-ingénierie

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Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

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Etablering af blodhjernebarrieremonokultur i μSiM-enhed til immuncytokemisk farvning og konfokal analyse

Journal JoVE
Bio-ingénierie

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Journal JoVE Bio-ingénierie

Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis

Etablering af blodhjernebarrieremonokultur i μSiM-enhed til immuncytokemisk farvning og konfokal analyse

Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

DOI:

10.3791/200520-v

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05:25 min

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January 12, 2024

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Molly C. McCloskey, Pelin Kasap, Michelle Trempel, Louis P. Widom, Julia Kuebel, Kaihua Chen, Thomas R. Gaborski, Britta Engelhardt, James L. McGrath

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

Tags

Blood-brain Barrier Monoculture SiM Device Immunocytochemistry Confocal Analysis Cell Culture Collagen Fibronectin HECSR EECM-BMEC Cell Detachment Endothelial Medium Methanol Fixation PBS Washing Blocking Primary Antibody Secondary Antibody Hoechst Staining

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