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Recherche en cancérologie

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TMEM200A: A Novel Oncogene Regulating EMT and PI3K/AKT in Gastric Cancer

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Proteindetektion i TMEM200A siRNA-transfekterede adenokarcinomceller i maven ved hjælp af Western Blotting

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Recherche en cancérologie

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Journal JoVE Recherche en cancérologie

Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting

Proteindetektion i TMEM200A siRNA-transfekterede adenokarcinomceller i maven ved hjælp af Western Blotting

TMEM200A: A Novel Oncogene Regulating EMT and PI3K/AKT in Gastric Cancer

DOI:

10.3791/200744-v

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02:49 min

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September 15, 2023

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Yaowen Zhang, Shanshan Kuang, Hancheng Qin, Na Zhao, Yixin Yang, Jisheng Xie

¹Department of Histology and Embryology,Youjiang Medical University for Nationalities, ²Department of Pathophysiology,Youjiang Medical University for Nationalities

Tags

TMEM200A SiRNA Stomach Adenocarcinoma Western Blotting Protein Detection Cell Culture PBS Radioimmune Precipitation Assay BCA Protein Assay SDS-PAGE PVDF Membrane TBST Blocking Buffer Primary Antibody Secondary Antibody ECL Substrate Epithelial Mesenchymal Transition Phosphoinositide 3-kinase Signaling Pathway

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