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Recherche en cancérologie

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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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Forberedelse af plade til fluorescerende billeddannelse og cellelevedygtighedsvurdering i CAR-Jurkat-cokultur med CFSE-mærkede tumorceller

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Recherche en cancérologie

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Journal JoVE Recherche en cancérologie

Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells

Forberedelse af plade til fluorescerende billeddannelse og cellelevedygtighedsvurdering i CAR-Jurkat-cokultur med CFSE-mærkede tumorceller

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

DOI:

10.3791/200793-v

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04:00 min

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October 27, 2023

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs, Jacqueline Babai, Riza Alker, Andrew K. Godwin^5,6, David Akhavan^2,3

¹Department of Radiation Oncology,University of Kansas Cancer Center, ²Department of Cancer Biology,University of Kansas Cancer Center, ³BioEngineering Program,University of Kansas, ⁴Department of Pathology and Laboratory Medicine,University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine,University of Kansas Medical Center

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Keywords: Fluorescent Imaging Cell Viability CAR-Jurkat Co-culture CFSE-labeled Tumor Cells Propidium Iodide Triton X Cell Imaging CFSE Fluorescence Cell Diameter Live/dead Cell Analysis Cell Counting Effector-to-target Ratio EGFR-targeting CAR Cytotoxicity PBMC-derived CD3 T Cells

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