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Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

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Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs)

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Biologie

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Journal JoVE Biologie

Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs)

Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs)

Efficient Rat Bone Marrow Neutrophil Extracellular Trap Isolation

DOI:

10.3791/200812-v

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02:06 min

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April 26, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang³, Zhenghua Xiao⁴, Li He⁴, Chaoyi Qin⁴

¹Department of Cardiovascular Surgery, West China Hospital,Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

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Keywords: Fluorometric Quantification Cytometric Analysis Rat Neutrophil Extracellular Traps (NETs)Tris-EDTA Buffer DNA Standards Assay Reagent Fluorescence Microplate Reader Nucleus Stain Cell-free DNA Stain Cytometer SYTOX Green HEXT Channel PMA Stimulation Cell-free DNA Content

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