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Biologie du développement

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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

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Arricchimento FACS della sospensione di cellule intestinali di zebrafish per scRNAseq

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Biologie du développement

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Journal JoVE Biologie du développement

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq

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Arricchimento FACS della sospensione di cellule intestinali di zebrafish per scRNAseq

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/200861-v

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01:10 min

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November 10, 2023

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Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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Keywords: FACS Gut Cell Suspension ScRNAseq Zebrafish Cell Sorting Flow Cytometry Cell Collection Cell Viability Single-cell RNA Sequencing

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