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Biologie du développement

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Detection and Quantification of Single mRNA Molecules in Drosophila

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Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization(단일 분자 RNA 형광 현장 교잡)을 위한 초파리의 날개 디스크 및 성인 근육 해부 및 준비

Journal JoVE
Biologie du développement

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Journal JoVE Biologie du développement

Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization

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Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization(단일 분자 RNA 형광 현장 교잡)을 위한 초파리의 날개 디스크 및 성인 근육 해부 및 준비

Detection and Quantification of Single mRNA Molecules in Drosophila

DOI:

10.3791/200894-v

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03:46 min

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September 08, 2023

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Emma Leroux, Nourhene Ammar, Savannah Moinet, Thierry Pecot, Hadi Boukhatmi

¹Institut de Génétique et Développement de Rennes (IGDR),UMR6290 CNRS – Université de Rennes, ²BIOSIT, UAR 3480 US 018,Université de Rennes

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Keywords: Drosophila Wing Discs Adult Muscles Single-molecule RNA FISH Dissection Paraformaldehyde Ethanol PBS PBT Hybridization Probe Antibody DAPI Mounting Medium

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Dissection and Preparation of Wing Discs and Adult Muscles from Drosophila for Single-Molecule RNA Fluorescence In Situ Hybridization(단일 분자 RNA 형광 현장 교잡)을 위한 초파리의 날개 디스크 및 성인 근육 해부 및 준비

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