/

/

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

/

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Journal JoVE
Biologie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biologie

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Please note that all translations are automatically generated. Click here for the English version.

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/201002-v

•

04:10 min

•

June 23, 2023

•

Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

Tags

Keywords: Transfection HeLa Cells CENP-E Knockout Plasmid Trypsin-EDTA Cell Passage Reduced Serum Medium Transfection Reagent Fluorescence Microscopy Cell Counting Serial Dilution Phenotype-based Screening DNA Extraction PCR Ligation Transformation Sanger Sequencing Wild-type Mutant

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Generazione di linee cellulari knockout CENP-E^-/- associate al centromero della proteina E utilizzando il sistema CRISPR/Cas9

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Read Article