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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

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Préparation de cellules synchronisées G1 pour mesurer la formation de foyers d’ADNss lors d’un stress génotoxique

Journal JoVE
Biologie

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Journal JoVE Biologie

Preparation of G1 Synchronized Cells to Measure ssDNA Foci Formation upon Genotoxic Stress

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Préparation de cellules synchronisées G1 pour mesurer la formation de foyers d’ADNss lors d’un stress génotoxique

Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

DOI:

10.3791/201062-v

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07:11 min

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December 22, 2023

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Qingyue Zhang², Marc A. Kerzhnerman², Nelson García-Vázquez^2,3, Gergely Rona^2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

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Here Are The Keywords From The Given Text: – G1 Synchronized Cells – SsDNA Foci Formation – Genotoxic Stress – Cell Synchronization – DNA Damage – DNA Repair

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