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Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
Journal JoVE
Biologie
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Journal JoVE Biologie
Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

DOI:
10.3791/2012-v

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05:45 min

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April 26, 2010

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, , ,
1Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Department of Physics, Lewis-Sigler Institute for Integrative Genomics,Princeton University

Chapitres

  • 00:00Titre
  • 00:41Introduction
  • 01:12Attaching Bacteria to a Coverlip and Preparing the Beads
  • 03:19Measuring the Bending Stiffness of the Bacterial Cells
  • 05:11Measuring the Bending Stiffness of Bacterial Cells Representative Results
  • 05:21Conclusion
  • 05:44Credits

Summary

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We present a protocol for bending filamentous bacterial cells attached to a cover-slip surface with an optical trap to measure the cellular bending stiffness.

Tags

MeasuringBending StiffnessBacterial CellsOptical TrapProtocolFilamentous Rod-shaped BacteriaForcesMicroscopic Three-dimensional SpringHigh-intensity Laser BeamChemically-treated CoverslipLive BacteriaGrowing EndsFilamentous GrowthDrug CephalexinCell AttachmentBending ForcesPolylysine-coated BeadForce MeasurementDisplacement Measurement

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