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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

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Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy

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Biologie

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Journal JoVE Biologie

Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy

Creating the Navigation Map for CLEM and Preparation of the Ultrathin Sections for Electron Microscopy

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

DOI:

10.3791/201226-v

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02:38 min

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January 12, 2024

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Shuyuan Wang*¹, Haiyan Xiong*^1,2, Qiyuan Chang*^1,3, Xudong Zhuang*^4,5,6, Yaochen Wu³, Xinrui Wang^5,6, Congxian Wu, Zhifei Fu^7,8

¹Public Technology Service Center,Fujian Medical University, ²The School of Pharmacy,Fujian Medical University, ³The School of Basic Medical Sciences,Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁵Medical Research Center,Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience,Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

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Keywords: Navigation Map Ultrathin Sections Electron Microscopy MScarlet Annulus Marker Bright Field Imaging Fluorescent Imaging Stitching Cells Of Interest Fluorescent Images Bright Field Images Mounting Buffer Double Distilled Water Pioloform Film Slot Grid Paraform

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