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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

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Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy

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Biologie

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Journal JoVE Biologie

Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy

Image Analysis of Brightfield and Fluorescence Images of the Mouse Brain Sections for Epon Post-Embedding Correlative Light and Electron Microscopy

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

DOI:

10.3791/201227-v

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02:13 min

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January 12, 2024

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Shuyuan Wang*¹, Haiyan Xiong*^1,2, Qiyuan Chang*^1,3, Xudong Zhuang*^4,5,6, Yaochen Wu³, Xinrui Wang^5,6, Congxian Wu, Zhifei Fu^7,8

¹Public Technology Service Center,Fujian Medical University, ²The School of Pharmacy,Fujian Medical University, ³The School of Basic Medical Sciences,Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁵Medical Research Center,Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience,Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

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Keywords: Image Analysis Brightfield Fluorescence Mouse Brain Epon Post-embedding Correlative Light And Electron Microscopy MScarlet ImageJ Stitching Sum Intensity Projection Gold Nanoparticles Merge Channels RGB Color

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