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Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

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蛍光顕微鏡画像と電子顕微鏡の相関光・電子顕微鏡画像のレジストレーション

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Biologie

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Journal JoVE Biologie

Registration of the Fluorescence Microscopy Image with the Electron Microscopy Image for Correlative Light and Electron Microscopy

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蛍光顕微鏡画像と電子顕微鏡の相関光・電子顕微鏡画像のレジストレーション

Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

DOI:

10.3791/201228-v

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02:30 min

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January 12, 2024

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Shuyuan Wang*¹, Haiyan Xiong*^1,2, Qiyuan Chang*^1,3, Xudong Zhuang*^4,5,6, Yaochen Wu³, Xinrui Wang^5,6, Congxian Wu, Zhifei Fu^7,8

¹Public Technology Service Center,Fujian Medical University, ²The School of Pharmacy,Fujian Medical University, ³The School of Basic Medical Sciences,Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁵Medical Research Center,Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience,Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

Tags

Keywords: Fluorescence Microscopy Electron Microscopy Correlative Light And Electron Microscopy Image Registration EasyCLIMv0 Non-rigid Alignment Gold Nanoparticles ImageJ Image Processing Composite Image Image Channels

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