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Immunologie et infection

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Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers

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Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers (Video) | JoVE

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Immunologie et infection

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Journal JoVE Immunologie et infection

Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers

Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers (Video) | JoVE

Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers

DOI:

10.3791/201233-v

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03:14 min

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January 19, 2024

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J. Thomas Gebert, Francesca J. Scribano, Kristen A. Engevik, Joseph M. Hyser

¹Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology,Baylor College of Medicine

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Keywords: Live Imaging Virus-infected Human Intestinal Organoid Monolayers Epifluorescence Microscope Stage Top Incubator Bright Field Illumination Fluorescent Calcium Sensor Fluorescently Tagged Viruses Amino Fluorescent Staining Formaldehyde Ammonium Chloride Triton X-100 Blocking Solution Primary Antibodies Secondary Antibodies DAPI Rotavirus Infection HIO Monolayer

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