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Bio-ingénierie

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Fabrication Followed by 3D Encapsulation of Tumor Spheroids in PEG Hydrogels

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Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

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Bio-ingénierie

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Journal JoVE Bio-ingénierie

Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

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Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

Fabrication Followed by 3D Encapsulation of Tumor Spheroids in PEG Hydrogels

DOI:

10.3791/201261-v

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03:35 min

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September 22, 2023

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Joseph Bruns, Shabnam Nejat, Allison Faber, Silviya P. Zustiak

¹Biomedical Engineering Department,Saint Louis University

Tags

Immunofluorescence Fixation Staining Clearing Encapsulated Spheroids Confocal Microscopy Hydrogel PBS Fixative Primary Antibodies Nestin SOX2 Formamide Z-stack Maximum Projection DAPI Stem Cell Marker

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