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Journal
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Immunology and Infection
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Prøveklargjøring for flowcytometri for å måle pHluorin2-fluorescens i levende
trypanosoma brucei
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living
Trypanosoma brucei
Prøveklargjøring for flowcytometri for å måle pHluorin2-fluorescens i levende
trypanosoma brucei
DOI:
10.3791/201368-v
•
00:54 min
•
January 19, 2024
•
Daniel Call*
1
,
Sabrina S. Pizarro*
2,3
,
Erica Tovey
,
Emily Knight
3
,
Carrie Baumgardner
4
,
Kenneth A. Christensen
,
James C. Morris
1
Department Chemistry and Biochemistry
,
Brigham Young University
,
2
Eukaryotic Pathogens Innovation Center
,
Clemson University
,
3
Department of Genetics and Biochemistry
,
Clemson University
,
4
Department of Physics and Astronomy
,
Clemson University
Tags
Flow Cytometry
PHluorin2
Trypanosoma Brucei
Sample Preparation
Tetracycline
Doxycycline
Cell Culture
Centrifugation
PBS
Glucose
Propidium Iodide
Thiazole Red
Live/dead Staining
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