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Immunologie et infection

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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

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Analyse af flowcytometridata til måling af glykosomets relative pH i levende trypanosoma brucei

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Immunologie et infection

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Journal JoVE Immunologie et infection

Analysis of Flow Cytometry Data to Measure the Relative pH of the Glycosome in Living Trypanosoma brucei

Analyse af flowcytometridata til måling af glykosomets relative pH i levende trypanosoma brucei

Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

DOI:

10.3791/201370-v

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04:00 min

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January 19, 2024

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Daniel Call*¹, Sabrina S. Pizarro*^2,3, Erica Tovey, Emily Knight³, Carrie Baumgardner⁴, Kenneth A. Christensen, James C. Morris

¹Department Chemistry and Biochemistry,Brigham Young University, ²Eukaryotic Pathogens Innovation Center,Clemson University, ³Department of Genetics and Biochemistry,Clemson University, ⁴Department of Physics and Astronomy,Clemson University

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Flow Cytometry Trypanosoma Brucei Glycosome PH Fluorescence Gating Singlets Live Cells PHL Positive

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