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One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
Journal JoVE
Biologie
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Journal JoVE Biologie
One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

DOI:
10.3791/2014-v

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09:28 min

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June 25, 2010

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1Edward A. Doisy Department of Biochemistry and Molecular Biology,Saint Louis University School of Medicine

Chapitres

  • 00:04Titre
  • 00:55Introduction
  • 01:58Removal of Urea from Urine Sample
  • 04:05Dehydration & Derivatization then GC/MS
  • 07:14Representative Results
  • 08:12Conclusion

Summary

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The urease method of sample preparation for GC/MS analysis of intermediary metabolites is presented by its inventor. The method allows one-step follow-up of newborn screening for inborn errors by tandem mass spectrometry by quantifying carbohydrates, organic and amino acids all in a single process.

Tags

One-step MetabolomicsCarbohydratesOrganic AcidsAmino AcidsTandem Mass SpectrometryAcylcarnitinesOrganic AcidemiasAmino Acid QuantificationEnzymatic TestingCarbohydrate DisordersUrease MethodSample PreparationGas ChromatographyDehydrationDerivatizationTrimethylsilylationCatalystTriethylammonium TrifluoroacetateAutomated InjectionChromatographyQuantification Of MetabolitesSemi-quantificationMass SpectraTMS Derivatized Biological Compounds

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