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Validating Xanthatin-Keap1 Interaction with Docking and CETSA
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Cell Culture and Cellular Thermal Shift Assay (CETSA) Sample Preparation for Western Blotting
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Médecine
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Journal JoVE Médecine
Cell Culture and Cellular Thermal Shift Assay (CETSA) Sample Preparation for Western Blotting

Cell Culture and Cellular Thermal Shift Assay (CETSA) Sample Preparation for Western Blotting

Validating Xanthatin-Keap1 Interaction with Docking and CETSA

DOI:
10.3791/201410-v

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02:35 min

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February 23, 2024

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, , , ,
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine

Tags

Cell CultureCETSAWestern BlottingSample PreparationRAW 264.7 CellsDMEMPBSCentrifugationLiquid NitrogenWater BathXanthatinDMSOSDS-PAGEProtein Loading Buffer

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