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Dot-Blot Assay for Detection of Leptospira DNA in Water Samples
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DNA-Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples
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Biologie
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Journal JoVE Biologie
DNA-Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples

DNA-Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples

Dot-Blot Assay for Detection of Leptospira DNA in Water Samples

DOI:
10.3791/201581-v

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01:29 min

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June 14, 2024

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, , , , ,
1Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, 2Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, 3Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Tags

Keywords: DNA-Membrane FixationProbe HybridisationLeptospira DetectionWater SamplesNylon MembraneDot BlotSodium HydroxideSSPEUltraviolet LightHybridization TubePre-hybridization SolutionLabeled Probe

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