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Dot-Blot Assay for Detection of Leptospira DNA in Water Samples

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Visualization of the DNA Hybrids Using Chemiluminescence for Leptospira Detection in Water Samples

Journal JoVE
Biologie

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Journal JoVE Biologie

Visualization of the DNA Hybrids Using Chemiluminescence for Leptospira Detection in Water Samples

Visualization of the DNA Hybrids Using Chemiluminescence for Leptospira Detection in Water Samples

Dot-Blot Assay for Detection of Leptospira DNA in Water Samples

DOI:

10.3791/201582-v

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02:15 min

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June 14, 2024

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Eduardo Delgadillo-Tellez, Beatriz Zavaleta-Villa, Daniel Atilano-López, Rigoberto Hernández-Castro, Angélica Olivo-Díaz, Erika Margarita Carrillo-Casas

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Tags

DNA Hybridization Chemiluminescence Leptospira Detection Water Samples PCR Amplification Nylon Membrane Probe Hybridization SSPE Buffer SDS Antibody Incubation Buffer Washing CSPD Radiographic Plate Leptospiral DNA Dot Blot Analysis

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