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Identifying Drugs that Affect Polymerization of Bacterial Cytoskeletal Proteins
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Schizosaccharomyces pombe'de İfade Edilen GFP Etiketli Bakteriyel Hücre İskeleti Proteinlerinin Hedeflenmesi ve Polimerlerin Mikroskobik Görüntülenmesi
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Biologie
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Journal JoVE Biologie
Targeting GFP-Tagged Bacterial Cytoskeletal Proteins Expressed in Schizosaccharomyces pombe and Microscopic Imaging of the Polymers
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Schizosaccharomyces pombe'de İfade Edilen GFP Etiketli Bakteriyel Hücre İskeleti Proteinlerinin Hedeflenmesi ve Polimerlerin Mikroskobik Görüntülenmesi

Identifying Drugs that Affect Polymerization of Bacterial Cytoskeletal Proteins

DOI:
10.3791/201596-v

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04:40 min

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April 26, 2024

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, , ,
1School of Biological Sciences,National Institute of Science Education and Research, 2Homi Bhabha National Institutes

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Keywords: GFP-taggedBacterial Cytoskeletal ProteinsSchizosaccharomyces PombeMicroscopic ImagingPolymersEMM (Edinburgh Minimal Medium)Concanavalin AEpifluorescence Microscopy96-well PlateAdaptive Auto-focusDIC (Differential Interference Contrast)Fluorescence ImagingZ-stack

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Schizosaccharomyces pombe'de İfade Edilen GFP Etiketli Bakteriyel Hücre İskeleti Proteinlerinin Hedeflenmesi ve Polimerlerin Mikroskobik Görüntülenmesi

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