Journal
/
Immunologie et infection
/
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

DOI:
10.3791/2017-v

•

08:39 min

•

June 01, 2010

•

,
1Center for Vaccine Biology and Immunology,University of Rochester

Chapitres

  • 00:00Titre
  • 00:37Introduction
  • 01:31Isolation of Human T Lymphocytes
  • 02:57Culture of Human T Lymphocytes
  • 05:04In Vitro Lymphocyte Migration Assay
  • 06:15Capturing an Image Sequence Using NIS Elements Software
  • 07:20Representative Results
  • 08:12Conclusion

Summary

Traduction automatique

Traduction automatique

T lymphocyte migration occurs during homing to lymphoid organs, exit from the vasculature, and entering into peripheral tissues. Here, we describe a protocol that can be used to analyze T lymphocyte migration in vitro.

Tags

T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2017?article_type=v

piggyBac Transposon System Ændring af primære humane T-celler
check_url/fr/2017?article_type=v

Lymfocyt Isolering fra menneskehud for Fænotypisk Analyse og<em> Ex vivo</em> Cell Culture
check_url/fr/2017?article_type=v

Mus Naïve CD4<sup> +</sup> T Cell Isolation og<em> In vitro</em> Differentiering til T-celledelmængder
check_url/fr/2017?article_type=v

Imaging CD4 T-celle Interstitiel Migration i det betændte Dermis
check_url/fr/2017?article_type=v

Flowcytometrisk analyse of Natural Killer Cell lytisk aktivitet i humant fuldblod
check_url/fr/2017?article_type=v

Isolering og flowcytometrisk analyse for Human endocervikale Gamma Delta T-celler
check_url/fr/2017?article_type=v

Analyse af lymfocyt Ekstravasation Ved anvendelse af en<em> In vitro</em> Model af humant blod-hjernebarrieren
check_url/fr/2017?article_type=v

Kvantificering af humane monocyt Chemotaxis In Vitro og Murine lymfocyt handel In Vivo
check_url/fr/2017?article_type=v

Isolation og In Vitro kultur af Murine og menneskelige alveolær makrofager
check_url/fr/2017?article_type=v

Analyse af Shear Flow-induceret Migration af Murine Marginal Zone B-celler In Vitro

Read Article