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A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
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Journal JoVE Biologie
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

DOI:
10.3791/2020-v

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06:59 min

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August 11, 2010

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, , ,
1Department of Surgery,Weill Cornell Medical College of Cornell University

Chapitres

  • 00:07Titre
  • 00:59Introduction
  • 01:55Validation of Morpholinos
  • 04:23Co-Injection of Morpholinos
  • 04:54Evaluation of Phenotypes
  • 05:37Representative Reuslts
  • 06:24Conclusion

Summary

Traduction automatique

Traduction automatique

Gene function can be obscured in loss-of-function experiments if there is compensation by another gene. The zebrafish model provides a relatively high-throughput means to reveal such functional redundancy in living embryos.

Tags

Reverse Genetic ApproachFunctional RedundancyEmbryogenesisLoss-of-function ExperimentsTargeted MutagenesisKnockoutZebrafish ModelGene-specific Antisense MorpholinosMRNATranslation InhibitionSplicing InhibitionKnockdownGene CompensationGene FamiliesSister GenesCo-expressionHigh-throughput TestingGenetic InteractionSub-threshold LevelsGATA Transcription Factor

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