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Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
DOI:

08:56 min

August 01, 2010

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Chapitres

  • 00:04Titre
  • 00:56Introduction
  • 01:44Preparation of hESC Pellet
  • 03:51Renal Capsule Grafting
  • 06:23Teratoma Harvest, Fixation, and Sectioning
  • 07:21Representative Images of Teratoma Sections
  • 08:25Conclusion

Summary

Traduction automatique

Directed differentiation of hESCs into specific cells has generated much interest in regenerative medicine. We provide a concise, step-by-step protocol for determining the in vivo fate of selected hESCs that provides a valuable tool for characterizing tissue-specific reagents for cell-based therapy.

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